PURPOSE:
The purpose of this lab was to find out how many parents of yours had a specific gene, called Alu. This gene does nothing, but it is still an interesting lab.
PORPOISE:
A small toothed whale with a low triangular dorsal fin and a blunt rounded snout.
HYPOTHESIS:
I hypothesize that I will get a +/+ result, which would mean that both of my parents have Alu.
MATERIALS:
In this lab, we used:
PROCEDURE:
First, we swished around saline in our mouths to get our DNA. We then put some of this saline solution in to a PCR tube, and spun it in a micro-centrifuge. Then we rack the newly formed cell pellet and add it to Chelex. Then we added a cap lock and heated it overnight. Next we took off the cap lock and put the PCR tube in a micro-centrifuge for another minute. We then transferred the supernatant from the Chelex/DNA tube to a new tube, and refrigerated it. next we put some of it in a small tube, and added Master Mix and Primer Mix. We put it in a thermal cycler and again waited overnight. The next day, we spun it in a micro-centrifuge, added loading dye, and put it in a hole in the gel, which was inside of a special gel box. We sent 150 volts through it for about 30 minutes, and then stained and photographed.
RESULTS:
I am heterozygous (+/-), which means that one of my parents has Alu while the other does not. I was one of two people who are heterozygous in my group.
ANALYSIS: My hypothesis was incorrect. This was expected, as it was less of a hypothesis and more of an uneducated guess.
The purpose of this lab was to find out how many parents of yours had a specific gene, called Alu. This gene does nothing, but it is still an interesting lab.
PORPOISE:
A small toothed whale with a low triangular dorsal fin and a blunt rounded snout.
HYPOTHESIS:
I hypothesize that I will get a +/+ result, which would mean that both of my parents have Alu.
MATERIALS:
In this lab, we used:
- 10 mL saline solution
- Cheek cells
- A cup
- 1.5 mL microfuge tube
- a microcentrifuge
- a microfuge tube rack
- Chelex tube
- Thermal cycler
- P-200 Micropipette
- a refrigerator
- Small PCR tube
- P-20 Micropipette
- 20 milliliters of Primer mix
- 5 milliliters of loading dye
- Gel box
- Gel
- Gel comb
PROCEDURE:
First, we swished around saline in our mouths to get our DNA. We then put some of this saline solution in to a PCR tube, and spun it in a micro-centrifuge. Then we rack the newly formed cell pellet and add it to Chelex. Then we added a cap lock and heated it overnight. Next we took off the cap lock and put the PCR tube in a micro-centrifuge for another minute. We then transferred the supernatant from the Chelex/DNA tube to a new tube, and refrigerated it. next we put some of it in a small tube, and added Master Mix and Primer Mix. We put it in a thermal cycler and again waited overnight. The next day, we spun it in a micro-centrifuge, added loading dye, and put it in a hole in the gel, which was inside of a special gel box. We sent 150 volts through it for about 30 minutes, and then stained and photographed.
RESULTS:
I am heterozygous (+/-), which means that one of my parents has Alu while the other does not. I was one of two people who are heterozygous in my group.
ANALYSIS: My hypothesis was incorrect. This was expected, as it was less of a hypothesis and more of an uneducated guess.